Re: Stage micrometers and diatoms

Posted by Julio Vazquez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Stage-micrometers-and-diatoms-tp589976p589980.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

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Shalin, 

When we purchased our Zeiss confocal, we got several test slides from Zeiss; these include a grid pattern of 2 micron wide (If I recall correctly) lines at 100 micron spacing, and a grating of 0.5 micron lines at 1 micron spacing (alternating dark and light 0.5 micron lines). In both cases, the lines are made of opaque/reflective thin metal deposit, I believe. These work well for measuring magnification and resolution/contrast. I am not sure where Zeiss gets them, or whether they sell them independently of their confocals, but if you have a contact for a Zeiss rep, might be worth asking them...

Another source of micrometers is Applied Image Inc...   they cost around $ 400.00. I think they call them "scales"

http://www.aig-imaging.com/mm5/merchant.mvc

Carolina Biological Supply company has several conventional stage micrometers for about $ 30.00-50.00. Don't know if any of those work in reflected light...

Google "Carolina Biological" , and then search for micrometer

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Julio Vazquez, 

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

[hidden email]

http://www.fhcrc.org/

On Aug 10, 2007, at 5:37 AM, Shalin Mehta wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear confocalists,

 Is there a stage micrometer that works in both transmission and reflection mode- for measuring true magnification? I have diatom slide from Mr. Klaus Kemp with 8 diatoms that allow evaluation of resolution. Are some features on diatom frustule known to have certain distance between them? Following diatoms are mounted on the slide.

• Amphipleura pellucida
• Frustulia rhomboides
• Pleurosigma angulatum
• Surirella gemma
• Nitzschia sigma
• Stauroneis phoenicentron
• Navicula lyra
• Gyrosigma balticum

Best,
Shalin
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Shalin Mehta, Graduate student
Graduate Programme in Bioengineering, NUS, Singapore
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