Re: Stage micrometers and diatoms

Posted by Julio Vazquez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Stage-micrometers-and-diatoms-tp589976p589982.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear diatom lovers, 

below are the spacings of the features of the different species on Klaus Kemp's 8-form test plate. I believe I got this info from the Carolina Biological supply web site, when they used to sell the slide.

There also used to be an article by Dave Walker: Counting teh dots: Giving Microscopes a workout using diatom test slides at  

www.microscopy-uk.org.uk/mag/artoct99/dwdiatom.html    

the article seems to be currently inaccessible, possibly being revised

Julio. 

• Amphipleura pellucida 270 nm
• Frustulia rhomboides 300 nm
• Pleurosigma angulatum 525 nm
• Surirella gemma 500 nm
• Nitzschia sigma 440 nm
• Stauroneis phoenicentron 720 nm
• Navicula lyra 1250 nm
• Gyrosigma balticum 670 nm

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

[hidden email]

http://www.fhcrc.org/

On Aug 10, 2007, at 5:37 AM, Shalin Mehta wrote:

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear confocalists,

 Is there a stage micrometer that works in both transmission and reflection mode- for measuring true magnification? I have diatom slide from Mr. Klaus Kemp with 8 diatoms that allow evaluation of resolution. Are some features on diatom frustule known to have certain distance between them? Following diatoms are mounted on the slide.

• Amphipleura pellucida
• Frustulia rhomboides
• Pleurosigma angulatum
• Surirella gemma
• Nitzschia sigma
• Stauroneis phoenicentron
• Navicula lyra
• Gyrosigma balticum

Best,
Shalin
--
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