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Re: Quantum yield

Posted by George McNamara on Aug 19, 2007; 12:25am
URL: http://confocal-microscopy-list.275.s1.nabble.com/Quantum-yield-tp590022p590023.html

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Hi Henriette,


Sort on the QY column of http://home.earthlink.net/~fluorescentdyes/McNamara2007FluorophoresTable.xls    
Best way to get the file is to go to
http://home.earthlink.net/~pubspectra/
scroll down to
McNamara 2007 Fluorophore Data Tables 
right click, and download to your computer.

If you just need one QY, go to the graphing website Carl set up http://www.mcb.arizona.edu/ipc/fret/index.html, select the dye, and click the notes icon (to the right of the dye dropdown after you've selected a dye). All the dyes on Carl's site are in the index file at
McNamara_Boswell_000_2006_Index _Dyes_FPs_Filters_Lamps_Other_Spectra.xls  
(column P of the McNamara Boswell Spectra Dyes worksheet - the Excel file opens to the lamp worksheet).


As pointed out by Martin, what you get in your experiment may not be the maximum possible QY. A trivial reason is that the solvent you are using may not be the solvent used in the measurement. For example, putting a lot of dyes on a protein can result in self-quenching, for examples DQ-collagen or Ralph Weissleder's many Cy5-protease substrates, or overloaded antibodies. for the same reason, fluorescent protein multimers (i.e. 5xGFP) are usually no brighter than monomers or dimers.



best wishes,


George
p.s. some listserv readers may find of interest:

McNamara G, Boswell C (2007) A Thousand Proteins of Light:  15 Years of Advances in Fluorescent Proteins. Modern Research and Educational Topics in Microscopy (volume 3), in press. http://www.formatex.org/microscopy3/     
you can try right clicking these two links in your email:  Word Doc Draft   Data
or go to http://home.earthlink.net/~pubspectra and download from there. Again, best to save to your computer rather than opening in a web browser.


At 07:27 AM 8/16/2007, you wrote:
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi all,

I am a new member of the confocal list, and I have a question regarding
quantum yield.

Does some kind of list exist with quantum yield of different probes, or
what is the easiest way to find out which probes are the brightest and
most stable ones. They all say they are “the best and brightest”.

Kind regards
Henriette




 

George McNamara, Ph.D.
University of Miami, Miller School of Medicine
Image Core
Miami, FL 33010
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305-243-8436 office