Posted by
George McNamara on
Aug 19, 2007; 12:25am
URL: http://confocal-microscopy-list.275.s1.nabble.com/Quantum-yield-tp590022p590023.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Henriette,
Sort on the QY column of
http://home.earthlink.net/~fluorescentdyes/McNamara2007FluorophoresTable.xls
Best way to get the file is to go to
http://home.earthlink.net/~pubspectra/
scroll down to
McNamara 2007 Fluorophore Data Tables
right click, and download to your computer.
If you just need one QY, go to the graphing website Carl set up
http://www.mcb.arizona.edu/ipc/fret/index.html, select the dye, and
click the notes icon (to the right of the dye dropdown after you've
selected a dye). All the dyes on Carl's site are in the index file at
McNamara_Boswell_000_2006_Index
_Dyes_FPs_Filters_Lamps_Other_Spectra.xls
(column P of the
McNamara Boswell Spectra Dyes worksheet -
the Excel file opens to the lamp worksheet).
As pointed out by Martin, what you get in your experiment may not be the
maximum possible QY. A trivial reason is that the solvent you are using
may not be the solvent used in the measurement. For example, putting a
lot of dyes on a protein can result in self-quenching, for examples
DQ-collagen or Ralph Weissleder's many Cy5-protease substrates, or
overloaded antibodies. for the same reason, fluorescent protein multimers
(i.e. 5xGFP) are usually no brighter than monomers or dimers.
best wishes,
George
p.s. some listserv readers may find of interest:
McNamara G, Boswell C (2007) A Thousand
Proteins of Light: 15 Years of Advances in Fluorescent Proteins.
Modern Research and
Educational Topics in Microscopy (volume 3), in press.
http://www.formatex.org/microscopy3/
you can try right clicking these two links in your email:
Word Doc Draft
Data
or go to
http://home.earthlink.net/~pubspectra and download from there. Again,
best to save to your computer rather than opening in a web browser.
At 07:27 AM 8/16/2007, you wrote:
Search the CONFOCAL archive
at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi all,
I am a new member of the confocal list, and I have a question regarding
quantum yield.
Does some kind of list exist with quantum yield of different probes, or
what is the easiest way to find out which probes are the brightest and
most stable ones. They all say they are “the best and
brightest”.
Kind regards
Henriette
George McNamara, Ph.D.
University of Miami, Miller School of Medicine
Image Core
Miami, FL 33010
[hidden email]
[hidden email]
305-243-8436 office