Re: Quantum yield

Posted by Martin Wessendorf on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Quantum-yield-tp590022p590029.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Henriette Madsen wrote:

> I am a new member of the confocal list, and I have a question regarding
> quantum yield.
>
> Does some kind of list exist with quantum yield of different probes, or
> what is the easiest way to find out which probes are the brightest and
> most stable ones. They all say they are “the best and brightest”.

Molecular Probes sometimes includes qualitative info on quantum yields.
  However, quantum yield is sensitive to environment.  The concentration
of fluorophore (and the fluorophore-to-protein ratio of a conjugate such
as a labeled antibody), the solvent surrounding the fluorophore, the
solvent's viscosity, the presence or absence of agents affecting
photobleaching, etc. can all affect the probability that a photon will
be emitted from an excited molecule.  Thus the quantum yield can be
different depending upon the conditions.

My own thought would just to try different labels and see what works
best for your system.

Good luck!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu