Re: Tracking with Metamorph

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URL: http://confocal-microscopy-list.275.s1.nabble.com/Tracking-with-Metamorph-tp590031p590034.html

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Dear Nishigandha-
If you can get MetaMorph to segment the cell boundary, even if it is
not perfect, it can then be instructed to calculate the centroid
positions.  These are the x,y coordinates of the ellipse of
concentration of the segmented figure.  The Euclidean distance
between any two centroids is a fair measure of the net displacement
of the cell.  This is if you are working on a two-dimensional plane.
In three dimensions, you would have to take the z dimension into
account.
Carol

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear All,
>
>We are trying to analyze cell motility using Metamorph
>software. We could not find the way to measure the net
>displacement of the cell, i.e., the straight line
>distance between the first point to the last point of
>the cell track traced. I will be thankful if anybody
>could help.
>
>Regards,
>
>Nishigandha Naik
>
>
>
>      
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--
Carol A. Heckman, Ph.D.
Director, Center for Microscopy & Microanalysis
and Professor of Biological Sciences
Bowling Green State University
Bowling Green, OH 43403
USA
website: http://www.bgsu.edu/departments/biology/facilities/MnM