http://confocal-microscopy-list.275.s1.nabble.com/Shift-in-GFP-spectrum-tp590043p590045.html
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Dear list,
>
>
>
> I have been performing spectral analysis experiments on pig-hearts
> injected with GFP-transfected stemcells with the Zeiss LSM510 META to
> define whether GFP was present or not. 1 to 3 days after injection, a
> spectral curve similar to GFP with a clear peak around 510 nm can be
> observed, though when scanning hearts 6 weeks after injection (with the
> same settings of microscope, laser and detectors), I only observe a
> broader spectral curve with a peak around 540nm, thoug with almost the
> same intensity. Since it is very crucial for the project to know
> whether the stemcells are still present or not after these 6 weeks, I
> want to make sure that the spectrum I observe is (not) GFP.
>
>
>
> So the question: is it possible GFP has a spectral shift of about 30nm,
> and gets a broader spectral curve, due to e.g. change in pH, presence of
> other proteines or whatever other influences? In addition, if so, is
> there any good reference available to this phenomenon?
>
>
>
> Thanks in advance,
>
>
>
> Sven Terclavers
>
>
>
>
>
> Disclaimer:
http://www.kuleuven.be/cwis/email_disclaimer.htm for more
> information.