Single Q-dots for PSF measurement

Posted by Lauran Oomen on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Confocal-List-on-Nabble-tp590120p590123.html

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Hello all,

 

We have tried to use Q-dots for PSF measurements on our confocals, but in our hands they are not bright enough and also bleach. I guess a number of you have tried Q-Dots as well, since they promise to be good sub-resolution targets. I have searched the archive and only found a message about this dating back to 2004, also reporting negative results. Did anyone successfully use Q-dots as sub-resolution targets to measure the PSF of a confocal? If yes, could you share your protocol with us?

 

Lauran

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Lauran Oomen
The Netherlands Cancer Institute
Digital Microscopy Facility (H0)
PO box 90203
1006BE Amsterdam
The Netherlands
tel: [phone]
fax: [phone]