Re: Single Q-dots for PSF measurement

Posted by Eli Rothenberg on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Confocal-List-on-Nabble-tp590120p590125.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You can suppress blinking, and have long lived surface
attached single Qdots in solution- should be no problem.
What's good about Qdots that you can excite them with any
wavelength below their emission.
Here are several references, for blinking suppression of dyes
and Qdots, and for localization using single fluorophores.
1)Hyokeun Park, George Hanson, Steven Duff, Paul Selvin. Nanometer Localization of Single ReAsH Molecules. Journal of Microscopy, 216, 199-205(2004).
2)Rasnik, I., S. A. McKinney, and T. Ha, "Nonblinking and Long-lasting Single Molecule Fluorescence Imaging", Nature Methods 3(11), 891-893 (2006)
3)Hohng S., and T. Ha, "Near-complete Suppression of Quantum Dot Blinking in Ambient Conditions", J. Am. Chem. Soc. 126(5), 1324-1325 (2004)

good luck

Eli

_______________
Eli Rothenberg
Postdoctoral research associate
HHMI lab and the
department of physics
University of Illinois
Urbana-Champaign
 

---- Original message ----

>Date: Thu, 30 Aug 2007 11:25:05 +0200
>From: Lauran Oomen <[hidden email]>  
>Subject: Single Q-dots for PSF measurement  
>To: [hidden email]
>
>   Search the CONFOCAL archive at
>   http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>   Hello all,
>    
>   We have tried to use Q-dots for PSF measurements
>   on our confocals, but in our hands they are
>   not bright enough and also bleach. I guess a number
>   of you have tried Q-Dots as well, since they promise
>   to be good sub-resolution targets. I have searched
>   the archive and only found a message about this
>   dating back to 2004, also reporting negative
>   results. Did anyone successfully use Q-dots as
>   sub-resolution targets to measure the PSF of a
>   confocal? If yes, could you share your protocol with
>   us?
>    
>   Lauran
>
>   *************************************
>   Lauran Oomen
>   The Netherlands Cancer Institute
>   Digital Microscopy Facility (H0)
>   PO box 90203
>   1006BE Amsterdam
>   The Netherlands
>   tel: [phone]
>   fax: [phone]