Re: Single Q-dots for PSF measurement

Posted by Matt Bootman on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Confocal-List-on-Nabble-tp590120p590126.html

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<vendor response>

Try a sample of  QD nanoclusters from Crystalplex. Nanoclusters are
aggregates of multiple QD's encased in a water soluble shell. They are
approx. 25 nm (+/-10 nm) in diameter. They are much brighter than individual
quantum dots and the signal from multiple dots averages out blinking.

Matt

Matt Bootman
CEO
Crystalplex Corporation
890 William Pitt Way
Pittsburgh, PA 15238
http://www.crystalplex.com
412.826.3081
412.826.3083 fx
724.344.7128 cell

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Eli Rothenberg
Sent: Thursday, August 30, 2007 12:25 PM
To: [hidden email]
Subject: Re: Single Q-dots for PSF measurement

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You can suppress blinking, and have long lived surface
attached single Qdots in solution- should be no problem.
What's good about Qdots that you can excite them with any
wavelength below their emission.
Here are several references, for blinking suppression of dyes
and Qdots, and for localization using single fluorophores.
1)Hyokeun Park, George Hanson, Steven Duff, Paul Selvin. Nanometer
Localization of Single ReAsH Molecules. Journal of Microscopy, 216,
199-205(2004).
2)Rasnik, I., S. A. McKinney, and T. Ha, "Nonblinking and Long-lasting
Single Molecule Fluorescence Imaging", Nature Methods 3(11), 891-893 (2006)
3)Hohng S., and T. Ha, "Near-complete Suppression of Quantum Dot Blinking in
Ambient Conditions", J. Am. Chem. Soc. 126(5), 1324-1325 (2004)

good luck

Eli

_______________
Eli Rothenberg
Postdoctoral research associate
HHMI lab and the
department of physics
University of Illinois
Urbana-Champaign
 

---- Original message ----

>Date: Thu, 30 Aug 2007 11:25:05 +0200
>From: Lauran Oomen <[hidden email]>  
>Subject: Single Q-dots for PSF measurement  
>To: [hidden email]
>
>   Search the CONFOCAL archive at
>   http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>   Hello all,
>    
>   We have tried to use Q-dots for PSF measurements
>   on our confocals, but in our hands they are
>   not bright enough and also bleach. I guess a number
>   of you have tried Q-Dots as well, since they promise
>   to be good sub-resolution targets. I have searched
>   the archive and only found a message about this
>   dating back to 2004, also reporting negative
>   results. Did anyone successfully use Q-dots as
>   sub-resolution targets to measure the PSF of a
>   confocal? If yes, could you share your protocol with
>   us?
>    
>   Lauran
>
>   *************************************
>   Lauran Oomen
>   The Netherlands Cancer Institute
>   Digital Microscopy Facility (H0)
>   PO box 90203
>   1006BE Amsterdam
>   The Netherlands
>   tel: [phone]
>   fax: [phone]