http://confocal-microscopy-list.275.s1.nabble.com/Re-pre-chirping-what-diagnostics-to-use-tp590162p590171.html
your needs. You will be able to to tune to the desired wavelength and
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear Jerry and
> others,
>
> What diagnostics do you use for tuning of two photon laser or OPO? Is
> laser spectrum analyzer enough?
>
> We have a shared two photon (MIRA 900F Femtosecond configuration) and
> OPO from Coherent. One of the main user is very afraid of tuning it -
> so he always has a service engineer tune it to 830nm and longest
> possible pulse (since he does spectroscopy). There are two more of us
> who will benefit by tuning it for two-photon and SHG (different
> wavelengths and shortest possible pulse).
> How is the 'reproducibility' of laser beam profile when working with
> these lasers? One of the main concern that other user has is that - if
> you cannot come back to exactly 830 nm after tuning to say 740 nm, the
> custom optical setup will go out of alignment.
>
> Thanks
> Regards
> Shalin
>
>
>
> On 8/30/07, *Mark Cannell* <
[hidden email]
> <mailto:
[hidden email]>> wrote:
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Hi Gerry
>
> The typical path using 2 prisms is via a small mirror into the first
> prism (we set this at minimum at minimum deviation but this can be
> changed to alter chirp). Then to the second prism (at minimum devation
> to produce a collimated horizontally spread spectrum), and then two
> mirrors to reflect the light back and displace the beam vertically
> which
> then passes back through the second and first prisms respectively.
> After
> the beam has left the first prism, note the beam has been displaced
> vertically is and thereby separated from the input beam.
>
> BUT are you sure you need to do this? If the input pulse is ~120
> fs the
> pulse broadening is quite minor. We used a pair of gold mirrors to to
> return and displace the beam but if you have 1" fs mirrors they would
> work as well.
>
> For typical prism spacing see:
>
> Soeller, C. & Cannell, M.B. (1996) Construction of a two-photon
> microscope and optimisation of illumination pulse width. _Pflugers
> Archiv_ 432: 555-561.
>
> Cheers Mark
>
> Jerry Sedgewick wrote:
> > Search the CONFOCAL archive at
> >
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> >
> > I am interested in setting up pre-chirping in the laser path
> going to
> > a custom built multiphoton. I have been searching in vain for exact
> > information on the components. I bought the prisms, but I am
> uncertain
> > about the first mirror that directs the laser to the prisms (and
> then
> > allows the returning, reflected laser to pass through), and the
> second
> > mirror. My guess is that the first mirror must be a linear
> polarizer,
> > or a polarizer that separates the s and p polarization. The second
> > mirror must be a grating of some sort that re-polarizes the laser so
> > that it passes through the first mirror.
> >
> > Rather than guessing, it would make my day if I could get
> specifics on
> > the mirrors.
> >
> > Thanks!
> >
> > Jerry Sedgewick
>
>
>
>
> --
> My co-ordinates:
> Shalin Mehta, Graduate student
> Graduate Programme in Bioengineering, NUS, Singapore
> Email: shalin {dot} mehta {at} gmail {dot} com
> Blog: electricsbm.blogspot.com <
http://electricsbm.blogspot.com>
> Mobile: +65 90694182