How to measure objective transmission curves?

Posted by Michael Weber-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-to-measure-objective-transmission-curves-tp590172.html

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Dear all,

one of the main differences of objectives is their transmission
efficiency at certain wavelengths. One way to compare this is the
trial-and-error method, however, this is not straight forward.

My idea is to use a combination of spectrophotometer and a lamp with a
more or less even spectra (i.e. Xenon) on some kind of optical bench.
This would make the setup independent from the manufacturer. Beside the
distance between the light source and the detector, there are obviously
more things to consider: different diameter of the back focal plane,
different focal lenghts...

I would like to hear about your opinion about how to measure objective
transmission. Have you ever done this in your lab? Did you find a setup
that worked for you?

cheers,
Michael