Re: How to measure objective transmission curves?

Posted by Andrew Resnick on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-to-measure-objective-transmission-curves-tp590172p590174.html

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We've done similar measurements, it's not too difficult.

The main trick is handling the large NA lenses.  Our setup was light
source -> objective -> integrating sphere -> spectrometer.  After
normalizing to the source, we obtained really good data.  It's pretty
easy, actually.

Andy


At 08:45 AM 8/31/2007, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear all,
>
>one of the main differences of objectives is their transmission
>efficiency at certain wavelengths. One way to compare this is the
>trial-and-error method, however, this is not straight forward.
>
>My idea is to use a combination of spectrophotometer and a lamp with
>a more or less even spectra (i.e. Xenon) on some kind of optical
>bench. This would make the setup independent from the manufacturer.
>Beside the distance between the light source and the detector, there
>are obviously more things to consider: different diameter of the
>back focal plane, different focal lenghts...
>
>I would like to hear about your opinion about how to measure
>objective transmission. Have you ever done this in your lab? Did you
>find a setup that worked for you?
>
>cheers,
>Michael

Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)