Re: How to measure objective transmission curves?

Posted by Andrew Resnick on
URL: http://confocal-microscopy-list.275.s1.nabble.com/How-to-measure-objective-transmission-curves-tp590172p590215.html

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Jens,

I could not find the data- I must have left it behind.  I don;t
recall anything odd; the transmission curves were uniformly high in
the visible and dropped off to about 50%, IIRC, at 1064 nm (the laser
tweezer wavelength).

Andy

At 10:04 AM 8/31/2007, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear Michael & Andy,
>
>For Olympus lenses you can look it up on the web...the info for other
>manufacturers lenses would be interesting though; do you plan to publish
>the numbers? Andy, would you mind to disclose yours?
>
>regards, jens
>
>---
>Dr. Jens Rietdorf
>Head Microscopy
>Novartis Research Foundation
>Friedrich-Miescher-Institute, wro1066.2.32
>Maulbeerstr.66, CH-4058 Basel, Switzerland
>phone [phone] mobil [phone]
>Email:rietdorf(at)fmi.ch
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[hidden email]] On
>Behalf Of Andrew Resnick
>Sent: Freitag, 31. August 2007 17:00
>To: [hidden email]
>Subject: Re: How to measure objective transmission curves?
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>We've done similar measurements, it's not too difficult.
>
>The main trick is handling the large NA lenses.  Our setup was light
>source -> objective -> integrating sphere -> spectrometer.  After
>normalizing to the source, we obtained really good data.  It's pretty
>easy, actually.
>
>Andy
>
>
>At 08:45 AM 8/31/2007, you wrote:
> >Search the CONFOCAL archive at
> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >Dear all,
> >
> >one of the main differences of objectives is their transmission
> >efficiency at certain wavelengths. One way to compare this is the
> >trial-and-error method, however, this is not straight forward.
> >
> >My idea is to use a combination of spectrophotometer and a lamp with a
> >more or less even spectra (i.e. Xenon) on some kind of optical bench.
> >This would make the setup independent from the manufacturer.
> >Beside the distance between the light source and the detector, there
> >are obviously more things to consider: different diameter of the back
> >focal plane, different focal lenghts...
> >
> >I would like to hear about your opinion about how to measure objective
> >transmission. Have you ever done this in your lab? Did you find a setup
>
> >that worked for you?
> >
> >cheers,
> >Michael
>
>Andrew Resnick, Ph. D.
>Instructor
>Department of Physiology and Biophysics
>Case Western Reserve University
>216-368-6899 (V)
>216-368-4223 (F)

Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)