Re: Estimating field of view in an LSM

Posted by Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Estimating-field-of-view-in-an-LSM-tp590288p590293.html

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Craig,

for the theory I don't know, but to actually meassure the field of
view (or the size of your pixels) you can use a 1951 USAF Resolution
Test Chart. See e.g.
http://en.wikipedia.org/wiki/1951_USAF_Resolution_Test_Chart

There are several suppliers you can google for. I got mine from
Edmund Optics. It has a mirror coating on the top surface so that it
can be used in reflection mode.

Steffen



At 22:16 20.09.2007, you wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Just to clarify, I am doing multiphoton with a single PMT, so I'm
>trying to measure the field that the laser is focused over at the
>focal plane of the objective for a given input beam deflection.
>
>Craig
>
>On 9/20/07, Craig Brideau <[hidden email]> wrote:
> > I was wondering how I estimate the field of view for a given lens in a
> > laser scanning microscope?  I know it will depend on the entrance
> > angle of the beam into the back aperture of the lens, but I don't know
> > how to translate this into lateral focal point displacement for a
> > given working distance/NA/magnification of lens.  Can anyone point me
> > towards some good references?
> >
> > Thanks,
> >
> > Craig
> >