Posted by
Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Estimating-field-of-view-in-an-LSM-tp590288p590295.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalYeah, I want to be able to predict the field ahead of time BEFORE I
buy an objective. I'll have to keep looking in to the theory. If it
was a simple lens I could just use some ray trace equations, but
microscope objectives are very much 'black box' devices due to their
complexity, and proprietary design. Still, good advice for
characterizing my existing lenses.
Thanks,
Craig
On 9/21/07, Steffen Dietzel <
[hidden email]> wrote:
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Craig,
>
> for the theory I don't know, but to actually meassure the field of
> view (or the size of your pixels) you can use a 1951 USAF Resolution
> Test Chart. See e.g.
>
http://en.wikipedia.org/wiki/1951_USAF_Resolution_Test_Chart>
> There are several suppliers you can google for. I got mine from
> Edmund Optics. It has a mirror coating on the top surface so that it
> can be used in reflection mode.
>
> Steffen
>
>
>
> At 22:16 20.09.2007, you wrote:
> >Search the CONFOCAL archive at
> >
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal> >
> >Just to clarify, I am doing multiphoton with a single PMT, so I'm
> >trying to measure the field that the laser is focused over at the
> >focal plane of the objective for a given input beam deflection.
> >
> >Craig
> >
> >On 9/20/07, Craig Brideau <
[hidden email]> wrote:
> > > I was wondering how I estimate the field of view for a given lens in a
> > > laser scanning microscope? I know it will depend on the entrance
> > > angle of the beam into the back aperture of the lens, but I don't know
> > > how to translate this into lateral focal point displacement for a
> > > given working distance/NA/magnification of lens. Can anyone point me
> > > towards some good references?
> > >
> > > Thanks,
> > >
> > > Craig
> > >
>