Re: CFP/YFP

Posted by Michael Cammer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/CFP-YFP-tp590300p590304.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Another important question is the temporal and spatial resolution you
require.  Do you need to look at organelles in one yeast moving "real
time" or fields of COS, Hela or fibroblasts over many hours?

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Ok, do you want to do confocal or two photon?
> Can you afford an off-the-shelf system, or are you building your own
> from scratch, or do you have an existing system you could modify?
>
> Craig
>
>
> On 9/21/07, Lee, Park Joo <[hidden email]> wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hi Everyone,
>>
>> Please help. What could be a good experimental setup to do live cell
>> imaging
>> using CFP and YFP on two separate channels such excitation wavelength
>> and
>> emission filters?
>>
>> Park
>>
>

_________________________________________
Michael Cammer   http://www.aecom.yu.edu/aif/