Confocal Microscopy List

Re: FCS of proteins in solution

Posted by mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FCS-of-proteins-in-solution-tp590361p590363.html

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Can you add a large excess of non-fluorescent protein, such as BSA, to your solution to block the glass?

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of els vanstreels
Sent: Thursday, September 27, 2007 9:10 AM
To: [hidden email]
Subject: FCS of proteins in solution

Dear all,

We are trying to perform fluorescence correlation spectroscopy measurements on proteins in solution (PBS buffer). Our sample is on a standard n0. 1.5 coverglass placed on an inverted microscope. We are experiencing a lot of problems with adherence of the proteins to the glass surface, in a sense that the signal is decreasing rapidly after bringing the sample onto the glass. Is there a way to circumvent this problem, eg by applying some coating to the coverglass or performing the measurements in an other type of buffer?

Thanks for all suggestions,

Els

Dr. Ir. Els Vanstreels

Rega Institue for Medical Research

Minderbroedersstraat 10

3000 Leuven

Belgium

Tel.: +32-16332881

Fax.: +32-16332131

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