Re: FCS of proteins in solution

Posted by heckman@bgnet.bgsu.edu on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FCS-of-proteins-in-solution-tp590361p590364.html

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Els-
There is a protein in plasma (also in serum) that binds tightly to
glass.  The replacement series of proteins was worked out years
ago by Leo Vroman of the New York City VA Hospital, if you wish to
look up the data.

If you put a 10% solution of plasma or serum on the surface, it will
coat it completely and prevent or at least drastically limit the
adsorption of your fluorescent proteins.  See anybody who does
tissue culture, and they will have the supplies you need.
Carol