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Zucker.Robert on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nikon-C1si-tp590408p590409.html
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalWe are using the Nikon C1Si at the EPA in North Carolina.
The spectral accuracy of the machine is absolutely excellent . I believe
the company guarantees 1mm accuracy with a 2.5mm band width. We are
getting that accuracy with the machine. .
Upon checking the system with a MIDL lamp as described previously (
see references below) we have found excellent spectral accuracy between
400- 725nm.( see references below)
One of the major limitations that we have seen with this unit and also
with the Zeiss meta 510 is the decreased light throughput . This
creates images that are noisier than conventional confocal microscopes.
In our hands it appears you will need to have a bright sample to make
the spectral system work properly. ,
In my opinion, all confocal spectral system suffer from a lack of
adequate software to analyze the spectral data derived from them. Image
is important but the spectral data evaluation is critical when using a
spectral system.
It is important to remember that no confocal system is perfect. All
confocal systems have there advantages and disadvantages with regard to
hardware and software. Unfortunately, most of the time one is not aware
of all the limitations of a confocal until the unit is operational in
the laboratory.
These references will be helpful on evaluating spectral systems for
accuracy, reliability, and reproducibility. They are available as a pdf
file on request.
Zucker, R.M. Rigby P, Clements I; Salmon W, Chua, P. Reliability of
Confocal Spectral Imaging Systems: Use of Multispectral Beads.
Cytometry V71A: 174-189 2007 (COVER)
Zucker RM Confocal Slide Based System :Performance. Cytometry 2006
69 A 659-676..
Zucker RM Confocal Microscopy Slide Based Systems: Instability.
Cytometry 2006 69A 677-690.
Zucker RM and Lerner J: Wavelength and Alignment tests for Confocal
Imaging systems Microscopy Research and Technique . 68:5 307-319
(2005).
Lerner JL Zucker, R.M. Calibration and validation of spectroscopic
imaging: Cytometry 62A:8-34 2004
Bob
Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory.
Telephone: 919-541-1585 Fax: 919-541-4017
e-mail:
[hidden email]
Mail address: Reproductive Toxicology Division, MD 67
2525 E.NC Highway 54
Research Triangle Park, North Carolina, 27711
Shipping address: 2525 E.NC Highway 54
Durham, NC, 27713
Adrian Smith
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal On 16/11/2006, at 12:25 PM, George McNamara wrote:
Jeff Larson of Nikon had a nice write-up of the Nikon C1si
spectral confocal head in the August issue of Cytometry. The
C1si uses the same PMT array as the Zeiss META, but with
(presumably) more clever optics (split polarization states
for better throughput, 3 options for bandwidth vs range).
I'm interesting in hearing of people's experience with the Nikon
C1si.
I've not seen it discussed much on this list and I don't think
there have been any in Australia up until now.
Regards,
Adrian Smith
Centenary Institute, Sydney, Australia
Is there is really no one on this list using a C1si? (I've had no
replies to my previous email).
Regards,
Adrian Smith
Centenary Institute, Sydney, Australia