http://confocal-microscopy-list.275.s1.nabble.com/Nikon-C1si-tp590408p590444.html
In general CCDs are more sensitive that PMTs. I can't speak to the
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Will this system have the same sort of sensitivity as a multi-anode
> PMT system like the C1si? This PARISS system seems to use a CCD
> camera as its detector, which will not be as sensitive, if I
> understand correctly?
>
> Craig
>
> On 10/5/07, Robert Zucker <
[hidden email]> wrote:
>
>> Search the CONFOCAL archive at
>>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>>
>> Criag
>> If spectral analysis is the endpoint you may want to consider the PARISS
>> system from Lightform. (
http://www.lightforminc.com/) that can be
>> placed on a widefield microscope.
>> We are using the PARISS system to obtain spectral data --it has 1nm
>> resolution and has a spectrum from 400-900nm. I find it to be a great
>> asset in the laboratory,
>> It is far more sensitive and accurate that any confocal system that I
>> have seen.
>>
>> Contact Jeremy Lerner for details on his system.
>> best wishes
>> Bob
>>
>>
>>
>>
>>
>>
>>
>>
>> LightForm, Inc.,
>> 601 Route 206, Suite 26-479
>> Hillsborough NJ 08844
>> Tel: (908)281 9098
>> Email:
[hidden email]
>>
>>
>>
>>
>>
>>
>> .
>>
>> Robert M. Zucker, PhD
>> U.S. Environmental Protection Agency
>> Office of Research and Development
>> National Health and Environmental Effects Research Laboratory.
>> Telephone: 919-541-1585 Fax: 919-541-4017
>> e-mail:
[hidden email]
>>
>> Mail address: Reproductive Toxicology Division, MD 67
>> 2525 E.NC Highway 54
>> Research Triangle Park, North Carolina, 27711
>>
>> Shipping address: 2525 E.NC Highway 54
>> Durham, NC, 27713
>>
>>
>>
>>
>> Craig Brideau
>> <craig.brideau@G
>> MAIL.COM> To
>> Sent by:
[hidden email]
>> Confocal cc
>> Microscopy List
>> <CONFOCAL@LISTSE Subject
>> RV.BUFFALO.EDU> Re: Nikon C1si?
>>
>>
>> 10/05/2007 05:48
>> PM
>>
>>
>> Please respond
>> to
>> Confocal
>> Microscopy List
>> <CONFOCAL@LISTSE
>> RV.BUFFALO.EDU>
>>
>>
>>
>>
>>
>>
>> Search the CONFOCAL archive at
>>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>>
>> Thanks for your input! As in everything, there's a trade-off. Still,
>> for us the spectral data will be worth it.
>>
>> Craig
>>
>> On 10/5/07, Robert Zucker <
[hidden email]> wrote:
>>
>>> Search the CONFOCAL archive at
>>>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>>>
>>> Craig
>>> The efficiency of detection is much less in the spectral mode due to
>>>
>> the
>>
>>> design of the multianode detector. In addition as Kurt Thorn said the
>>> light is split into a number of different bandpass channels instead of
>>> being detected by only one channel which also limits the detection in
>>> each channel.
>>> Nikon compensates for this decreased light by increasing the pixel
>>>
>> dwell
>>
>>> time. However photons are photons. With less photons you will get a
>>> noisier image.
>>> The spectral detection of any confocal system using a multianode
>>> detector will not be as good as a PMT designed for good sensitivity
>>>
>> and
>>
>>> low light detection. It will produce nosier images but if there is
>>> enough light you will be able to determine a valuable spectrum which
>>>
>> can
>>
>>> be used for scientific experiments and to manipulate images. Like
>>> everything with confocal microscopy there is trade-offs and no perfect
>>> system.
>>> Best wishes.
>>> Bob
>>>
>>> Robert M. Zucker, PhD
>>> U.S. Environmental Protection Agency
>>> Office of Research and Development
>>> National Health and Environmental Effects Research Laboratory.
>>> Telephone: 919-541-1585 Fax: 919-541-4017
>>> e-mail:
[hidden email]
>>>
>>> Mail address: Reproductive Toxicology Division, MD 67
>>> 2525 E.NC Highway 54
>>> Research Triangle Park, North Carolina, 27711
>>>
>>> Shipping address: 2525 E.NC Highway 54
>>> Durham, NC, 27713
>>>
>>>
>>>
>>>
>>> Craig Brideau
>>> <craig.brideau@G
>>> MAIL.COM>
>>>
>> To
>>
>>> Sent by:
[hidden email]
>>> Confocal
>>>
>> cc
>>
>>> Microscopy List
>>> <CONFOCAL@LISTSE
>>>
>> Subject
>>
>>> RV.BUFFALO.EDU> Re: Nikon C1si?
>>>
>>>
>>> 10/04/2007 02:49
>>> PM
>>>
>>>
>>> Please respond
>>> to
>>> Confocal
>>> Microscopy List
>>> <CONFOCAL@LISTSE
>>> RV.BUFFALO.EDU>
>>>
>>>
>>>
>>>
>>>
>>>
>>> Search the CONFOCAL archive at
>>>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>>>
>>> I was wondering if you saw this decreased throughput in spectral mode
>>> only, or also in conventional detection mode? The spectral mode works
>>> with a multi-anode PMT, which has a lower quantum efficiency than a
>>> conventional PMT. From what I have seen of the design, it also has
>>> the option to use a conventional PMT system with the device, and I am
>>> wondering if you have also tried that mode of operation?
>>>
>>> Thanks,
>>>
>>> Craig
>>>
>>> On 10/4/07, Robert Zucker <
[hidden email]> wrote:
>>>
>>>> One of the major limitations that we have seen with this unit and
>>>>
>> also
>>
>>>> with the Zeiss meta 510 is the decreased light throughput . This
>>>> creates images that are noisier than conventional confocal
>>>>
>>> microscopes.
>>>
>>>> In our hands it appears you will need to have a bright sample to
>>>>
>> make
>>
>>>> the spectral system work properly. ,
>>>>
>
>