Confocal Microscopy List

Re: Nikon C1si?

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nikon-C1si-tp590408p590445.html

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So what's the catch? Why do people still use PMTs vs. CCDs or EMCCDs? Cost?

Craig

On 10/5/07, Kurt Thorn <[hidden email]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> In general CCDs are more sensitive that PMTs. I can't speak to the
> sensitivities of the systems here, but a typical PMT has a quantum
> efficiency on the order of 10 - 25%, whereas good CCDs have QEs from 60
> - 90%.
>
> Kurt
>
> Craig Brideau wrote:
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Will this system have the same sort of sensitivity as a multi-anode
> > PMT system like the C1si? This PARISS system seems to use a CCD
> > camera as its detector, which will not be as sensitive, if I
> > understand correctly?
> >
> > Craig
> >
> > On 10/5/07, Robert Zucker <[hidden email]> wrote:
> >
> >> Search the CONFOCAL archive at
> >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>
> >> Criag
> >> If spectral analysis is the endpoint you may want to consider the PARISS
> >> system from Lightform. ( http://www.lightforminc.com/) that can be
> >> placed on a widefield microscope.
> >> We are using the PARISS system to obtain spectral data --it has 1nm
> >> resolution and has a spectrum from 400-900nm. I find it to be a great
> >> asset in the laboratory,
> >> It is far more sensitive and accurate that any confocal system that I
> >> have seen.
> >>
> >> Contact Jeremy Lerner for details on his system.
> >> best wishes
> >> Bob
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >>
> >> LightForm, Inc.,
> >> 601 Route 206, Suite 26-479
> >> Hillsborough NJ 08844
> >> Tel: (908)281 9098
> >> Email: [hidden email]
> >>
> >>
> >>
> >>
> >>
> >>
> >> .
> >>
> >> Robert M. Zucker, PhD
> >> U.S. Environmental Protection Agency
> >> Office of Research and Development
> >> National Health and Environmental Effects Research Laboratory.
> >> Telephone: 919-541-1585 Fax: 919-541-4017
> >> e-mail: [hidden email]
> >>
> >> Mail address: Reproductive Toxicology Division, MD 67
> >> 2525 E.NC Highway 54
> >> Research Triangle Park, North Carolina, 27711
> >>
> >> Shipping address: 2525 E.NC Highway 54
> >> Durham, NC, 27713
> >>
> >>
> >>
> >>
> >> Craig Brideau
> >> <craig.brideau@G
> >> MAIL.COM> To
> >> Sent by: [hidden email]
> >> Confocal cc
> >> Microscopy List
> >> <CONFOCAL@LISTSE Subject
> >> RV.BUFFALO.EDU> Re: Nikon C1si?
> >>
> >>
> >> 10/05/2007 05:48
> >> PM
> >>
> >>
> >> Please respond
> >> to
> >> Confocal
> >> Microscopy List
> >> <CONFOCAL@LISTSE
> >> RV.BUFFALO.EDU>
> >>
> >>
> >>
> >>
> >>
> >>
> >> Search the CONFOCAL archive at
> >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>
> >> Thanks for your input! As in everything, there's a trade-off. Still,
> >> for us the spectral data will be worth it.
> >>
> >> Craig
> >>
> >> On 10/5/07, Robert Zucker <[hidden email]> wrote:
> >>
> >>> Search the CONFOCAL archive at
> >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>>
> >>> Craig
> >>> The efficiency of detection is much less in the spectral mode due to
> >>>
> >> the
> >>
> >>> design of the multianode detector. In addition as Kurt Thorn said the
> >>> light is split into a number of different bandpass channels instead of
> >>> being detected by only one channel which also limits the detection in
> >>> each channel.
> >>> Nikon compensates for this decreased light by increasing the pixel
> >>>
> >> dwell
> >>
> >>> time. However photons are photons. With less photons you will get a
> >>> noisier image.
> >>> The spectral detection of any confocal system using a multianode
> >>> detector will not be as good as a PMT designed for good sensitivity
> >>>
> >> and
> >>
> >>> low light detection. It will produce nosier images but if there is
> >>> enough light you will be able to determine a valuable spectrum which
> >>>
> >> can
> >>
> >>> be used for scientific experiments and to manipulate images. Like
> >>> everything with confocal microscopy there is trade-offs and no perfect
> >>> system.
> >>> Best wishes.
> >>> Bob
> >>>
> >>> Robert M. Zucker, PhD
> >>> U.S. Environmental Protection Agency
> >>> Office of Research and Development
> >>> National Health and Environmental Effects Research Laboratory.
> >>> Telephone: 919-541-1585 Fax: 919-541-4017
> >>> e-mail: [hidden email]
> >>>
> >>> Mail address: Reproductive Toxicology Division, MD 67
> >>> 2525 E.NC Highway 54
> >>> Research Triangle Park, North Carolina, 27711
> >>>
> >>> Shipping address: 2525 E.NC Highway 54
> >>> Durham, NC, 27713
> >>>
> >>>
> >>>
> >>>
> >>> Craig Brideau
> >>> <craig.brideau@G
> >>> MAIL.COM>
> >>>
> >> To
> >>
> >>> Sent by: [hidden email]
> >>> Confocal
> >>>
> >> cc
> >>
> >>> Microscopy List
> >>> <CONFOCAL@LISTSE
> >>>
> >> Subject
> >>
> >>> RV.BUFFALO.EDU> Re: Nikon C1si?
> >>>
> >>>
> >>> 10/04/2007 02:49
> >>> PM
> >>>
> >>>
> >>> Please respond
> >>> to
> >>> Confocal
> >>> Microscopy List
> >>> <CONFOCAL@LISTSE
> >>> RV.BUFFALO.EDU>
> >>>
> >>>
> >>>
> >>>
> >>>
> >>>
> >>> Search the CONFOCAL archive at
> >>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>>
> >>> I was wondering if you saw this decreased throughput in spectral mode
> >>> only, or also in conventional detection mode? The spectral mode works
> >>> with a multi-anode PMT, which has a lower quantum efficiency than a
> >>> conventional PMT. From what I have seen of the design, it also has
> >>> the option to use a conventional PMT system with the device, and I am
> >>> wondering if you have also tried that mode of operation?
> >>>
> >>> Thanks,
> >>>
> >>> Craig
> >>>
> >>> On 10/4/07, Robert Zucker <[hidden email]> wrote:
> >>>
> >>>> One of the major limitations that we have seen with this unit and
> >>>>
> >> also
> >>
> >>>> with the Zeiss meta 510 is the decreased light throughput . This
> >>>> creates images that are noisier than conventional confocal
> >>>>
> >>> microscopes.
> >>>
> >>>> In our hands it appears you will need to have a bright sample to
> >>>>
> >> make
> >>
> >>>> the spectral system work properly. ,
> >>>>
> >
> >
>
>
> --
> Kurt Thorn, PhD
> Director, Nikon Imaging Center
> University of California San Francisco
>
> UCSF MC 2140
> Genentech Hall Room S252
> 600 16th St.
> San Francisco, CA 94158-2517
>
> http://nic.ucsf.edu
> phone 415.514.9709
> fax 415.514.4300
>