Re: Nikon C1si?

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nikon-C1si-tp590408p590458.html

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No, the Zeiss has a 32-channel detector and I'm pretty sure

it is the same Hamamatsu unit.  It's just that they can only read

out 8 channels at once.  So you need 4 passes to read out all

32 channels.

 

                                                                          Guy

 

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis

     http://www.guycox.com/optical.htm
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Associate Professor Guy Cox, MA, DPhil(Oxon)
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From: Confocal Microscopy List on behalf of Carl Boswell
Sent: Fri 5/10/2007 10:49 AM
To: [hidden email]
Subject: Re: Nikon C1si?

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Hi Julio,

From my understanding, the Zeiss Meta has an 8-detector array, so more than one scan is needed if the desired bandwidth detection is greater than ~135 nm using the finest lamda resolution (10.7 nm).

Carl

 

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
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----- Original Message -----

From: [hidden email]

To: [hidden email]

Sent: Thursday, October 04, 2007 5:15 PM

Subject: Re: Nikon C1si?

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal -

We do not have a Nikon C1si, so can't answer your question specifically.  If anyone is interested though, I happened to wonder about the nature of the spectral detector, and Hamamatsu has a PMT Model number H7620 which is a 32-PMT array, that I suspect is very similar to the one used in the Zeiss META and possibly the C1si. (Zeiss would not say if this is the case...).  The detectors are 0.8 mm wide, with a center-to-center distance of 1 mm.  Specs can be downloaded from the Hamamatsu web site.

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 

http://www.fhcrc.org

==

On Oct 3, 2007, at 9:09 PM, Adrian Smith wrote:

I'm interesting in hearing of people's experience with the Nikon C1si. 

I've not seen it discussed much on this list and I don't think there have been any in Australia up until now. 

Regards,

Adrian Smith

Centenary Institute, Sydney, Australia

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