Re: Nikon C1si?

Posted by Kurt Thorn on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nikon-C1si-tp590408p590465.html

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I run a C1si at the Nikon Imaging Center at UCSF.  We use the spectral
mode relatively infrequently as most of our users are doing
straightforward three color imaging.  We have done some work with the
spectral mode, either for separating dyes with overlapping emission
spectra or for removing autofluorescence.  It seems to perform quite
well in spectral mode, although having accurately measured spectra is
important for good unmixing.  It is also a few fold slower in spectral
mode than conventional (3 PMT) mode.  Regarding the loss in intensity in
spectral mode mentioned by earlier, I would imagine part of this is due
to dividing the total light collected among multiple channels,
decreasing the light collected by each.

In the conventional, 3 PMT mode, where this system gets most of its use,
people like it very much.  The optics are very good and the brightness
and resolution of images have gotten a lot of compliments.

Kurt


Adrian Smith wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> On 16/11/2006, at 12:25 PM, George McNamara wrote:
>>
>>>
>>> Jeff Larson of Nikon had a nice write-up of the Nikon C1si spectral
>>> confocal head in the August issue of Cytometry. The C1si uses the
>>> same PMT array as the Zeiss META, but with (presumably) more clever
>>> optics (split polarization states for better throughput, 3 options
>>> for bandwidth vs range).
>>
>>
>> I'm interesting in hearing of people's experience with the Nikon C1si.
>>
>> I've not seen it discussed much on this list and I don't think there
>> have been any in Australia up until now.
>>
>> Regards,
>>
>> Adrian Smith
>> Centenary Institute, Sydney, Australia
>>
>>
>
>
>
> Is there is really no one on this list using a C1si?  (I've had no
> replies to my previous email).
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
>
>

--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

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