Posted by
Rosemary.White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/software-tp590473p590477.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalDera Carl,
Most imaging packages have "extended depth of focus" modules in them these
days.
We use the small (i.e. cheap) version of AutoMontage from Syncroscopy, which
does a reasonable job, and the license dongle can be moved between
computers. The expensive full version does a very good job but has to
remain on one computer, which can be a pain.
We've also tried the non-commercial CombineZ -
www.hadleyweb.pwp.blueyonder.co.uk/CZM/combinezm.htm, which is good too.
cheers,
Rosemary
Dr Rosemary White
[hidden email]
CSIRO Plant Industry ph. 61 (0)2-6246 5475
GPO Box 1600 fax. 61 (0)2-6246 5334
Canberra, ACT 2601
Australia
> From: Carl Boswell <
[hidden email]>
> Reply-To: Confocal Microscopy List <
[hidden email]>
> Date: Fri, 5 Oct 2007 16:34:55 -0700
> To: <
[hidden email]>
> Subject: software
>
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
> Dea all,
> Does anyone know of software that selects only in focus portions of an image
> and discards the out-of-focus parts. We need to get clear views of a curved
> surface (the fly eye) with a 10x objective using reflected light. Only thin
> optical slices are in focus at this mag, but if we could put together the
> portions that are in focus over the entire depth of the specimen, we could
> rebuild a clean image of the structure. This wouild be a used in an assay
> screening a bizillion flies, so SEM is not really practical.
> thanks,
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709