Re: software

Posted by Julio Vazquez on Oct 06, 2007; 1:11am
URL: http://confocal-microscopy-list.275.s1.nabble.com/software-tp590473p590478.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal --

Hi Carl, 

I think that's what the imageJ plugin "Stack Focuser" does.  Check it at at the imagej website:

http://rsb.info.nih.gov/ij/plugins/stack-focuser.html

I believe Metamorph, and possibly other software packages have similar tools, often called "Extended depth of focus" or similar

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

http://www.fhcrc.org/

On Oct 5, 2007, at 4:34 PM, Carl Boswell wrote:

Search the CONFOCAL archive at

http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dea all,

Does anyone know of software that selects only in focus portions of an image and discards the out-of-focus parts.  We need to get clear views of a curved surface (the fly eye) with a 10x objective using reflected light.  Only thin optical slices are in focus at this mag, but if we could put together the portions that are in focus over the entire depth of the specimen, we could rebuild a clean image of the structure.  This wouild be a used in an assay screening a bizillion flies, so SEM is not really practical.

thanks,

Carl

Carl A. Boswell, Ph.D.

Molecular and Cellular Biology

University of Arizona

520-954-7053

FAX [phone]