Re: software

Posted by Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/software-tp590473p590479.html

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Hi Carl,
I've used this plugin for ImageJ.  It does appear that one loses a  
few gray levels in the end result, at least with this plugin and a  
with commercial product to which it was compared.

http://rsb.info.nih.gov/ij/plugins/stack-focuser.html

Regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]

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The box said "Requires WindowsXP or better", so I bought a Macintosh.
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On Oct 5, 2007, at 4:34 PM, Carl Boswell wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dea all,
> Does anyone know of software that selects only in focus portions of  
> an image and discards the out-of-focus parts.  We need to get clear  
> views of a curved surface (the fly eye) with a 10x objective using  
> reflected light.  Only thin optical slices are in focus at this  
> mag, but if we could put together the portions that are in focus  
> over the entire depth of the specimen, we could rebuild a clean  
> image of the structure.  This wouild be a used in an assay  
> screening a bizillion flies, so SEM is not really practical.
> thanks,
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX [phone]