Re: software

Posted by Luke, Steve on
URL: http://confocal-microscopy-list.275.s1.nabble.com/software-tp590473p590480.html

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MetaMorph's 'Best Focus' projection does this.

Steve

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Carl Boswell
Sent: Friday, October 05, 2007 7:35 PM
To: [hidden email]
Subject: software

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Dea all,
Does anyone know of software that selects only in focus portions of an
image
and discards the out-of-focus parts.  We need to get clear views of a
curved
surface (the fly eye) with a 10x objective using reflected light.  Only
thin
optical slices are in focus at this mag, but if we could put together
the
portions that are in focus over the entire depth of the specimen, we
could
rebuild a clean image of the structure.  This wouild be a used in an
assay
screening a bizillion flies, so SEM is not really practical.
thanks,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX [phone]