Re: Mosaic with optical sectoning

Posted by Sven Terclavers on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mosaic-with-optical-sectoning-tp590526p590531.html

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Rachael,

I don't know if it's possible to install on your equipment, but I get nice
results with the Zeiss LSM510 software and their Multi Time 4.0 macro. Large
3D mosaics with multiple tracks (3 to 4 fluorophores) do not give problems,
unless the file is larger than 3Gb, but then it's not the software though
our Windows-version that blocks the use of more memory (a 64-bit system will
allow higher memory allocation, we run the 32-bit version).

It might be possible to create lsm-z-stack files from a z-stack saved as
single Tiff-files and then run the Mosaic tile-utility on those single
lsm-files, but this I've never tried.

Sven Terclavers

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Carol Heckman
Sent: Thursday, 18 October, 2007 01:36
To: [hidden email]
Subject: Re: [CONFOCAL] Mosaic with optical sectoning

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Rachaei-
I don't know where you are writing from, but you can do anything with
Advanced Visualization Systems software.  The learning curve is
pretty steep and they may not have it everywhere.

>  the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi! We are trying to run an experiment that requires image acquisition of a
>very large area (a slice of rat brain; we use mosaic for this purpose),
>along with optical sectioning.
>
>However, the software that we are currently using does not allow us to
>acquire both mosaic and optical sectioning simultaneously due to the
>extremely large file size produced (about 2GB), which cannot be processed

by

>the software (software limitation of file size is 200MB).
>
>I am wondering if it is possible for us to acquire the mosaic and optical
>sectioning separately (i.e. mosaic for every plane of interest), export out
>and compile them with another software. Does any of you have any suggestion
>of possible software that has the capability of compiling individual planes
>into a stack?
>
>Please advise. Thank you!
>
>Cheers,
>Rachael Lim

--
Carol A. Heckman, Ph.D.
Director, Center for Microscopy & Microanalysis
and Professor of Biological Sciences
Bowling Green State University
Bowling Green, OH 43403
USA
website: http://www.bgsu.edu/departments/biology/facilities/MnM



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