http://confocal-microscopy-list.275.s1.nabble.com/Mosaic-with-optical-sectoning-tp590526p590532.html
you may want to try out the software described in S.L. Wearne et al
summary of the paper). The process is done post acquisition.
>Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
>Hi! We are trying to run an experiment that requires image acquisition of a
>very large area (a slice of rat brain; we use mosaic for this purpose),
>along with optical sectioning.
>
>However, the software that we are currently using does not allow us to
>acquire both mosaic and optical sectioning simultaneously due to the
>extremely large file size produced (about 2GB), which cannot be processed by
>the software (software limitation of file size is 200MB).
>
>I am wondering if it is possible for us to acquire the mosaic and optical
>sectioning separately (i.e. mosaic for every plane of interest), export out
>and compile them with another software. Does any of you have any suggestion
>of possible software that has the capability of compiling individual planes
>into a stack?
>
>Please advise. Thank you!
>
>Cheers,
>Rachael Lim