coverslips for cell culture

Posted by Fredrik Wermeling on
URL: http://confocal-microscopy-list.275.s1.nabble.com/coverslips-for-cell-culture-tp590595.html

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Hi!

 

I’m planning to grow adherent cells for later staining with fluorescently labelled antibodies. Previously I’ve done this by adding a sterile round coverslip in a 12 well plate and kept the cells on the coverslip through out the staining and fixation and then just "mounted" them on a microscope slide. My questions are:

- Do you have any suggestions for which coverslips to purchase, I guess they have to be thin enough and without auto fluorescence.

- Is there any alternative? I saw that Nunc sells a Lab tek product that sounds like what I´m looking for. 

 

Thanks a lot

/ Fredrik