Re: coverslips for cell culture

Posted by Kurt Thorn on
URL: http://confocal-microscopy-list.275.s1.nabble.com/coverslips-for-cell-culture-tp590595p590596.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You want to use coverslips that are 0.17 mm thick - number 1.5
coverslips are the closest in thickness.

You can also use coverslip bottom plates or coverlsip bottom dishes -
these are very convenient if you want to image the cells live but may be
more of a pain for fixing and staining.  Glass-bottom-dishes.com sell a
number of different varieties.

Kurt

Fredrik Wermeling wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi!
>
>  
>
> I’m planning to grow adherent cells for later staining with
> fluorescently labelled antibodies. Previously I’ve done this by adding
> a sterile round coverslip in a 12 well plate and kept the cells on the
> coverslip through out the staining and fixation and then just
> "mounted" them on a microscope slide. My questions are:
>
> - Do you have any suggestions for which coverslips to purchase, I
> guess they have to be thin enough and without auto fluorescence.
>
> - Is there any alternative? I saw that Nunc sells a Lab tek product
> that sounds like what I´m looking for.
>
>  
>
> Thanks a lot
>
> / Fredrik
>
>  
>

--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

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