Re: super resolution - Zeiss enters the boat

Posted by Chris Wood-5 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/coverslips-for-cell-culture-tp590595p590610.html

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No surprise at all that Zeiss picked up this technique, no way they would
let Leica hog the field for long. But I can't see how they can get much
out of this, as it's basically an imaging protocol, rather than an
instrumentation advance. OK, you can package up the acquisition parameters
and post-processing steps in a module/macro, but there's nothing to stop
anyone executing this technique on any microscope with the minumum of
effort. And it seems to me that STED will be suitable for live cells
sooner than the PALM-STORM techniques.

This doesn't look much more than a spoiler tactic to me on the part of
Zeiss.

Saludos

Chris

>I think you're right.  Looks as if they've teamed up with the group
>headed by Eric Betzig.  He had the paper in Science last year:
>
>"Imaging Intracellular Fluorescent Proteins at Nanometer Resolution"
>Eric Betzig, George H. Patterson, Rachid Sougrat, O. Wolf Lindwasser,
>Scott Olenych, Juan S. Bonifacino, Michael W. Davidson, Jennifer
>Lippincott-Schwartz, and Harald F. Hess
>Science 15 September 2006 313: 1642-1645
>
>If that's it, it seems like a powerful technique, lower-cost than the
>Leica system, but unfortunately also very slow.  Also, it appears only
>applicable to photoactivatable fluorescent proteins (or dyes) that will
>change their spectrum to a longer wavelength when excited with a shorter
>wavelength.  It has the advantage of using much less intense
>illumination than the Leica system but I don't know of anyone using it
>for live-cell work.
>
>Martin
>--
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