Re: super resolution - Zeiss enters the boat

Posted by Martin Wessendorf on
URL: http://confocal-microscopy-list.275.s1.nabble.com/coverslips-for-cell-culture-tp590595p590623.html

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Michael Weber wrote:

> http://www.zeiss.de/C1256A770030BCE0/WebViewAllE/162CCB55A50A433FC12573A8002B17D9 

> The press release contains quite some side-kicks to Leica. And the
> technique sounds kind of STORM-like. Does somebody has further information?

I think you're right.  Looks as if they've teamed up with the group
headed by Eric Betzig.  He had the paper in Science last year:

"Imaging Intracellular Fluorescent Proteins at Nanometer Resolution"
Eric Betzig, George H. Patterson, Rachid Sougrat, O. Wolf Lindwasser,
Scott Olenych, Juan S. Bonifacino, Michael W. Davidson, Jennifer
Lippincott-Schwartz, and Harald F. Hess
Science 15 September 2006 313: 1642-1645

If that's it, it seems like a powerful technique, lower-cost than the
Leica system, but unfortunately also very slow.  Also, it appears only
applicable to photoactivatable fluorescent proteins (or dyes) that will
change their spectrum to a longer wavelength when excited with a shorter
wavelength.  It has the advantage of using much less intense
illumination than the Leica system but I don't know of anyone using it
for live-cell work.

Martin
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