Re: coverslips for cell culture

Posted by Sarah Aubut on
URL: http://confocal-microscopy-list.275.s1.nabble.com/coverslips-for-cell-culture-tp590595p590624.html

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I have used the Nunc chambered coverglass, and they have worked
excellent for me with viewing living cells and labeling them while doing
time lapse. These are like the chamber slide, except the bottom is the
coverglass and the chambers cannot be removed, which makes them perfect
for viewing live cells. Make sure you get the LabTek II, not the Labtek
I, as the II's have the 1.5 thickness. I order mine directly from
Nalgene-Nunc without a problem. The only issue I had was they are
shorter then a normal slide, so did not fit into my stage, but as I use
them all the time, I invested in a Prior stage that is designed for
them. I'm sure you can easily rig your normal stage if they don't fit.

Sarah Aubut
Lab Technician, Microscopy Suite
Canadian Forestry Service
Sault Ste Marie, ON
Canada

[hidden email]

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Glen H MacDonald
Sent: Friday, October 26, 2007 12:16 AM
To: [hidden email]
Subject: Re: coverslips for cell culture

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Erie Glass, which sells under their own label as well as sells to other
companies, actually owns Menzel.  
The glass sold by both is now made by Erie Electroverre in Romont,
Switzerland.   Erie has other plants
around the world and is now part of Thermo-Fisher.

How long before there are only 2 corporations left in the world?

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center Box 357923 University
of Washington Seattle, WA 98195-7923
[phone]

On Fri, 26 Oct 2007, Stephen Cody wrote:

> Search the CONFOCAL archive at
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>
> Dear Kurt,
>
> I suspect Menzel and Assistent  (Hecht) are the only manufacturers of
> coverslip glass. I suspect other
companies buy the glass, perhaps cut it, and rebrand it.  This is
certainly what Lomb in Australia do, they are quite open about it. If
there are other manufacturers of coverslip glass I'd be happy to be
corrected. So if you buy from a particular company or supplier, I
suspect is is probably Menzel or Assistent glass anyway, and you need to
put a little pressure on your supplier to contact their manufacturer and
order the correct thickness.

>
> Cheers
> Stephen H. Cody
> Microscopy Manager
> Central Resource for Advanced Microscopy Ludwig Institute for Cancer
> Research PO Box 2008 Royal Melbourne Hospital
> Parkville, Victoria,      3050
> Australia
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> ________________________________
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> From: Confocal Microscopy List on behalf of Kurt Thorn
> Sent: Fri 26/10/2007 9:42 AM
> To: [hidden email]
> Subject: Re: coverslips for cell culture
>
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Does anyone know of a US source for these?  When I this came up
> earlier on the list I did some searching for a US distributor but came

> up empty handed.  I agree that if you can get these it would be a big
> improvement on using #1.5 coverslips.
>
> Kurt
>> Both Menzel and Assistent (Hecht Glaswarenfabrik [Thanks Beat]) sell
>> a better standard thickness
for confocal microscopy it is called:

>> "170 +/- 0.01mm"
>>
>
> --
> Kurt Thorn, PhD
> Director, Nikon Imaging Center
> University of California San Francisco
>
> UCSF MC 2140
> Genentech Hall Room S252
> 600 16th St.
> San Francisco, CA 94158-2517
>
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