Re: coverslips for cell culture

Posted by Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/coverslips-for-cell-culture-tp590595p590625.html

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the Nunc II chambers are useful, but the thickness still varies.  I  
bought a blank insert for the our Prior stage and had it machined to  
take 60 mm coverslips in x dimension and Nunc chambers in the y  
direction.  A small trim of the corners at the intersection of the 2  
rectangular openings allows a 35 mm glass bottom dish to be used, as  
well.  a 1.5 cm semi-circle enlargement at the ends of the  
rectangular openings allow access to the lens to apply immersion media.

Glen

On Oct 26, 2007, at 4:53 AM, Aubut, Sarah wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I have used the Nunc chambered coverglass, and they have worked
> excellent for me with viewing living cells and labeling them while  
> doing
> time lapse. These are like the chamber slide, except the bottom is the
> coverglass and the chambers cannot be removed, which makes them  
> perfect
> for viewing live cells. Make sure you get the LabTek II, not the  
> Labtek
> I, as the II's have the 1.5 thickness. I order mine directly from
> Nalgene-Nunc without a problem. The only issue I had was they are
> shorter then a normal slide, so did not fit into my stage, but as I  
> use
> them all the time, I invested in a Prior stage that is designed for
> them. I'm sure you can easily rig your normal stage if they don't fit.
>
> Sarah Aubut
> Lab Technician, Microscopy Suite
> Canadian Forestry Service
> Sault Ste Marie, ON
> Canada
>
> [hidden email]
>
> -----Original Message-----
> From: Confocal Microscopy List  
> [mailto:[hidden email]] On
> Behalf Of Glen H MacDonald
> Sent: Friday, October 26, 2007 12:16 AM
> To: [hidden email]
> Subject: Re: coverslips for cell culture
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Erie Glass, which sells under their own label as well as sells to  
> other
> companies, actually owns Menzel.
> The glass sold by both is now made by Erie Electroverre in Romont,
> Switzerland.   Erie has other plants
> around the world and is now part of Thermo-Fisher.
>
> How long before there are only 2 corporations left in the world?
>
> Regards,
> Glen
>
> Glen MacDonald
> Core for Communication Research
> Virginia Merrill Bloedel Hearing Research Center Box 357923 University
> of Washington Seattle, WA 98195-7923
> [phone]
>
> On Fri, 26 Oct 2007, Stephen Cody wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear Kurt,
>>
>> I suspect Menzel and Assistent  (Hecht) are the only manufacturers of
>> coverslip glass. I suspect other
> companies buy the glass, perhaps cut it, and rebrand it.  This is
> certainly what Lomb in Australia do, they are quite open about it. If
> there are other manufacturers of coverslip glass I'd be happy to be
> corrected. So if you buy from a particular company or supplier, I
> suspect is is probably Menzel or Assistent glass anyway, and you  
> need to
> put a little pressure on your supplier to contact their  
> manufacturer and
> order the correct thickness.
>>
>> Cheers
>> Stephen H. Cody
>> Microscopy Manager
>> Central Resource for Advanced Microscopy Ludwig Institute for Cancer
>> Research PO Box 2008 Royal Melbourne Hospital
>> Parkville, Victoria,      3050
>> Australia
>> Tel: [phone]    Fax: [phone]
>> email: [hidden email]
>> www.ludwig.edu.au/labs/confocal.html
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>> ________________________________
>>
>> From: Confocal Microscopy List on behalf of Kurt Thorn
>> Sent: Fri 26/10/2007 9:42 AM
>> To: [hidden email]
>> Subject: Re: coverslips for cell culture
>>
>>
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Does anyone know of a US source for these?  When I this came up
>> earlier on the list I did some searching for a US distributor but  
>> came
>
>> up empty handed.  I agree that if you can get these it would be a big
>> improvement on using #1.5 coverslips.
>>
>> Kurt
>>> Both Menzel and Assistent (Hecht Glaswarenfabrik [Thanks Beat]) sell
>>> a better standard thickness
> for confocal microscopy it is called:
>>> "170 +/- 0.01mm"
>>>
>>
>> --
>> Kurt Thorn, PhD
>> Director, Nikon Imaging Center
>> University of California San Francisco
>>
>> UCSF MC 2140
>> Genentech Hall Room S252
>> 600 16th St.
>> San Francisco, CA 94158-2517
>>
>> http://nic.ucsf.edu <http://nic.ucsf.edu/> phone [phone]
>> fax   [phone]
>>