Re: coverslips for cell culture
Posted by
Donnelly, Tom on
URL: http://confocal-microscopy-list.275.s1.nabble.com/coverslips-for-cell-culture-tp590595p590627.html
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The Titertek II ( Nunc) 4 and 8 well dishes
have # 1.5 coverslip bottoms and they work well.
I would suggest looking at www.willcowells.com they make very
high quality coverslip bottom 35mm dishes with both 12mm and 22mm 170um
coverslips. They also make a "kit" which would allow you to grow cells on the
coverslip then insert it into the machined 35mm dish.
No commercial interest in either.
Tom
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Hi!
Im planning to grow adherent cells for later
staining with fluorescently labelled antibodies. Previously Ive done this by
adding a sterile round coverslip in a 12 well plate and kept the cells on the
coverslip through out the staining and fixation and then just "mounted"
them on a microscope slide. My questions are:
- Do you have any suggestions for
which coverslips to purchase, I guess they have to be thin enough and
without auto fluorescence.
- Is there any alternative? I saw that
Nunc sells a Lab tek product that sounds like what I´m looking
for.
Thanks a lot
/ Fredrik