Re: coverslips for cell culture
Posted by
Sandrine Pouvreau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/coverslips-for-cell-culture-tp590595p590628.html
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Hi.
I'm doing this kind of things with Hek
cells or myotubes on cell culture round dishes with glass coverslip bottom
of several kind. The coverslip has to be 0.17mm thin, but I never had any
autofluorescence problem. I'm for instance using (from Fisher Scientist)
Delta TPG dish, 0.17mm. They have a thick ring of plastic surrounding the
glass coverslip, so it is hard to focus on the cells that are on the edge,
but I just cut it with scissor and it works pretty well.
I wish you good luck
Sandrine
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Hi!
I’m planning to grow adherent cells for later staining
with fluorescently labelled antibodies. Previously I’ve done this by adding
a sterile round coverslip in a 12 well plate and kept the cells on the
coverslip through out the staining and fixation and then just "mounted"
them on a microscope slide. My questions are:
- Do you have any suggestions for which coverslips to
purchase, I guess they have to be thin enough and without auto fluorescence.
- Is there any alternative? I saw that Nunc sells a Lab
tek product that sounds like what I´m looking for.
Thanks a lot
/ Fredrik