Re: Objective for confocal

Posted by Nowell, Cameron on Oct 29, 2007; 11:13am
URL: http://confocal-microscopy-list.275.s1.nabble.com/Objective-for-confocal-tp590639p590644.html

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Magnification is increased with a 100x lens when compared to a 63x sure, but if you are using confocal then this isn't that important since you can digitally zoom in to get the same magnification. There are really no advantages of going to a 100x bjective if you have a high NA 63x available.
 
 
 
Cheers
 
 
 
Cam
 
 

Cameron Nowell B. Sc (Hons)

 

Microscopy Imaging and Research Core Facility

Peter MacCallum Cancer Centre

7 St Andrews Place

East Melbourne, Victoria 3002

AUSTRALIA

 

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________________________________

From: Confocal Microscopy List on behalf of Peter Pitrone
Sent: Mon 29/10/2007 8:24 PM
To: [hidden email]
Subject: Re: Objective for confocal


Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Magnification?!?!

On Oct 26, 2007, at 10:09 PM, Yang, Ann-Fook wrote:


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        Hi All,

        I am new in confocal microscopy.
        We have a 40x/1.3 oil and a 63X/1.2 water objectives. Is there any advantage to add a 100X/1.46 Oil objective?

        Ann Fook Yang,
        Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada
        EM Unit/ Unite EM
        Edifice K.W. Neatby Building,
        960 Carling Av /960 Boulevard Carling,
        Ottawa,Ontario
        K1A 0C6

        [hidden email]
        Telephone/Téléphone: 613-759-1638
        Facsimile/Télécopieur: 613-759-1701


Peter Gabriel Pitrone
Light Microscopy Facility
Max Planck Institute for
Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 Dresden, Germany
E-Mail: [hidden email]





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