Posted by
kspencer007 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Microinjection-system-tp590721.html
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHello all;
Slightly off topic, but I'd like to ask about microinjection
systems. We currently have an Eppendorf Transject (yes, it's old), and
are looking to replace it. The micromanipulator part (NI2) we have
demoed is fabulous. However, we are looking for more precise delivery of
small volumes in brain. We are doing cell tracing experiments in live
animals. The Eppendorf system (FemtoJet) has a great backflow pressure
compensator that works well in cultured cells, but not so well in
tissue. We have also tried their Cell Tram and Cell Tram Vario, but tend
to get a sudden gush of fluorescent dextran released inappropriately.
What we are dreaming of is a very fine controlled release of dye
precisely when we want it, in an extremely small volume. We realize that
the capillary size and diameter are the real factors here, and that
brain slices tend to clog the capillaries as you are moving through the
tissue. Would you know of a product at which we should look?
Any suggestions would be very welcome.
Thanks!
Kathy
Kathryn Spencer, Ph.D.
The Scripps Research Institute
ICND 210
10550 N. Torrey Pines Road
La Jolla, CA 92037
(858) 784-8437
[hidden email]