axial drift in confocal microscopy

Posted by vb-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Analytical-Quantitative-Light-Microscopy-Course-tp590728p590730.html

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Dear All,

 

I would greatly appreciate any reference to the careful evaluation of the axial drift (thermal, ... etc.) during the photobleaching step in FRAP experiments (e.g. on a single-, 4- or 9-voxel resolution level of, let say ca. 80x80x450 nm per voxel). Fluorescent beads (Q-dots) and fluorescently labeled virus particles in a live or a fixed cell could be a nice system for the cross-validation of this very important test.  

 

Thank you very much in advance,

 

Vitaly

NCI-Frederick,

301-846-6575