Re: Non-arc source for IX-81

Posted by Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FW-Deconvolve-1-42-Components-Setup-now-OK-tp590745p590776.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hello, you should also check out the new illuminator from Chroma. It is just like the Exfo only twice as powerful!

http://www.chroma.com/

 

 

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

1450 E Duarte Rd

Duarte, CA 91010

626-359-8111 x62872

http://www.cityofhope.org/SharedResources/LightMicroscopy

 


From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Julio Vazquez
Sent: Monday, November 05, 2007 12:35 PM
To: [hidden email]
Subject: Re: Non-arc source for IX-81

 

=

Hi Glen, 

 

Not an Olympus IX-81, but we have an EXFO 120W high-pressure mercury halide lamp for viewing fluorescence on a Zeiss LSM (Axiovert 200 stand). We like this lamp for several reasons:

 

1,000-1,5000 lifetime (typically, one lamp lasts 8-12 months; power starts to decline after ~ 1,000 hours, but lamp remains useable until about 1,500 hours), easy to install and does not require alignment, quite bright, built-in aperture that allows to control illumination intensity, removes heat source away from microscope (lamp is located inside power supply module and channeled through a fiber into the microscope), built-in safety device prevents turning lamp ON while still hot. 

 

We have not used this lamp for imaging, so I don't know if it is as bright or stable as a conventional mercury lamp, but it does look quite bright to me. We normally set the lamp aperture to 1-2 (out of five, with five being the brightest setting) for viewing samples. In terms of stability, it does show a decline over time, especially past 1,000 hours.

 

The system is maybe ~ $ 2,000 more than conventional mercury lamp system at purchase.  Lamps cost about $ 650.00, that is about $ 0.50 / hour... in the same ballpark as mercury lamps

 

 

Also, I should mention that Applied Precision now report using a 250 W Xenon  lamp on their DeltaVision system. Don't know any specifics, but I suppose they saw advantages compared to the HBO lamp.

 

Julio.

--

Julio Vazquez

Fred Hutchinson Cancer Research Center

Seattle, WA 98109-1024

 

 

 

 

 

On Nov 5, 2007, at 11:02 AM, Glen MacDonald wrote:



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Has anyone replaced the mercury illuminator on an Olympus IX-81 with a non-arc source such as metal-halogen or LED?  I'm looking for experiences regarding reliability, operational cost and performance on a laser scanning confocal installation.

 

Off-line commercial responses are welcome.

Thanks,

Glen

 

 

 

Glen MacDonald

Core for Communication Research

Virginia Merrill Bloedel Hearing Research Center

Box 357923

University of Washington

Seattle, WA 98195-7923  USA

(206) 616-4156

 

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The box said "Requires WindowsXP or better", so I bought a Macintosh.

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