Re: combination of 3 fluorescence protein reporters

Posted by Adrian Smith-6 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/combination-of-3-fluorescence-protein-reporters-tp590861p590863.html

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Note - evrogen are selling mKate as "TagFP635" (took me a while to  
spot that).

Also, isn't it somewhat shorter than Cy5? (mKate = excitation max 588,  
emission max 635)

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia



On 12/11/2007, at 1:13 AM, George McNamara wrote:

>
>
> If your microscope can image Cy5 well, try mKate from www.evrogen.com
>
>
>
> At 10:06 AM 11/8/2007, you wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear listers,
>>
>> One of our users wanted to image CFP and YFP labeled cells and  
>> would like to
>> add a third fluorecence protein reporter later. I was wondering if  
>> anybody
>> could make a recomendation on this third one.
>>
>> Anybody has ever used a  CFP/YFP/mCherry combination ?
>>
>> Thank you very much.
>>
>>
>> Xinyu Zhao
>> Biomedical Imaging Core Lab
>> School of Medicine
>> University of Pennsylvania
>> Tel:  [phone]
>
>
>
>
>
>
> George McNamara, Ph.D.
> University of Miami, Miller School of Medicine
> Image Core
> Miami, FL 33010
> [hidden email]
> [hidden email]
> 305-243-8436 office
> http://home.earthlink.net/~pubspectra/
> http://home.earthlink.net/~geomcnamara/
> http://www.sylvester.org/health_pro/shared_resources/index.asp (see  
> Analytical Imaging Core Facility)