Confocal Purchase...

Posted by Brain Jones on
URL: http://confocal-microscopy-list.275.s1.nabble.com/combination-of-3-fluorescence-protein-reporters-tp590861p590864.html


Adrian,

I see we have a tender now in process - you were building an RFP, did the situation change on your end?

We will proceed to submit the tender naturally but just wanted to touch base with you.

Kind Regards,
Brian

Brian Jones
Business Manager
Micro-Imaging Systems Group
Precision Instruments Division
Australia & New Zealand

Tel: [phone]
Mobil 0437 848 180

Adrian Smith <[hidden email]> Sent by: Confocal Microscopy List <[hidden email]> 12/11/2007 09:43 AM Please respond to Confocal Microscopy List <[hidden email]>

To [hidden email] cc Subject Re: combination of 3 fluorescence protein reporters

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Note - evrogen are selling mKate as "TagFP635" (took me a while to
spot that).

Also, isn't it somewhat shorter than Cy5? (mKate = excitation max 588,
emission max 635)

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia



On 12/11/2007, at 1:13 AM, George McNamara wrote:
>
>
> If your microscope can image Cy5 well, try mKate from www.evrogen.com
>
>
>
> At 10:06 AM 11/8/2007, you wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear listers,
>>
>> One of our users wanted to image CFP and YFP labeled cells and
>> would like to
>> add a third fluorecence protein reporter later. I was wondering if
>> anybody
>> could make a recomendation on this third one.
>>
>> Anybody has ever used a  CFP/YFP/mCherry combination ?
>>
>> Thank you very much.
>>
>>
>> Xinyu Zhao
>> Biomedical Imaging Core Lab
>> School of Medicine
>> University of Pennsylvania
>> Tel:  [phone]
>
>
>
>
>
>
> George McNamara, Ph.D.
> University of Miami, Miller School of Medicine
> Image Core
> Miami, FL 33010
> [hidden email]
> [hidden email]
> 305-243-8436 office
> http://home.earthlink.net/~pubspectra/
> http://home.earthlink.net/~geomcnamara/
> http://www.sylvester.org/health_pro/shared_resources/index.asp (see
> Analytical Imaging Core Facility)
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