Re: Confocal Purchase...
Posted by
Adrian Smith-6 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/combination-of-3-fluorescence-protein-reporters-tp590861p590865.html
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Hi Brian,
Yes - the procurement situation changed here due to several factors and as a result it was decided that we should use the University process for the confocal purchase.
From a technical point of view our requirements are as we have been discussing (and as I hope are captured in the tender documentation).
On 19/11/2007, at 11:51 AM, Brain Jones wrote:
Adrian,
I see we have a tender now in process - you were building an RFP, did the situation change on your end?
We will proceed to submit the tender naturally but just wanted to touch base with you.
Kind Regards,
Brian
Brian Jones
Business Manager
Micro-Imaging Systems Group
Precision Instruments Division
Australia & New Zealand
Tel: +61 03 9265 5419
Mobil 0437 848 180
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Note - evrogen are selling mKate as "TagFP635" (took me a while to
spot that).
Also, isn't it somewhat shorter than Cy5? (mKate = excitation max 588,
emission max 635)
Regards,
Adrian Smith
Centenary Institute, Sydney, Australia
On 12/11/2007, at 1:13 AM, George McNamara wrote:
>
>
> If your microscope can image Cy5 well, try mKate from www.evrogen.com
>
>
>
> At 10:06 AM 11/8/2007, you wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear listers,
>>
>> One of our users wanted to image CFP and YFP labeled cells and
>> would like to
>> add a third fluorecence protein reporter later. I was wondering if
>> anybody
>> could make a recomendation on this third one.
>>
>> Anybody has ever used a CFP/YFP/mCherry combination ?
>>
>> Thank you very much.
>>
>>
>> Xinyu Zhao
>> Biomedical Imaging Core Lab
>> School of Medicine
>> University of Pennsylvania
>> Tel: 215-898-6730
>
>
>
>
>
>
> George McNamara, Ph.D.
> University of Miami, Miller School of Medicine
> Image Core
> Miami, FL 33010
> [hidden email]
> [hidden email]
> 305-243-8436 office
> http://home.earthlink.net/~pubspectra/
> http://home.earthlink.net/~geomcnamara/
> http://www.sylvester.org/health_pro/shared_resources/index.asp (see
> Analytical Imaging Core Facility)
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