Leica resonant scanner-live for cell imaging

Posted by Kost, Benedikt on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Leica-resonant-scanner-live-for-cell-imaging-tp590925.html

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Can anyone compare the performance of the Leica SP5 resonant scanner with
that of a spinning disk (yokagawa) confocal system in terms of
- image quality
- photobleaching/phototxicity
- general "usefulness" for live cell confocal imaging

The resonant scanner was recently demonstrated to us and I was quite
impressed. I guess it is not as fast as spinning disk confocals can be, but
you seem to be able to image at a rate of at least 10 fps at 512x512, which
is all we need. The images we got were very nice, but  I did not have the chance to do extensive tests.

 

I would appreciate very much comments from anyone who has experience using the resonant scanner for live cell confocal imaging.

Many thanks,

Benedikt Kost
____________________________________________________

Benedikt Kost, PhD
Warwick HRI
Wellesbourne
Warwickshire, CV35 9EF
UK

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