Stability issues with LAS AF 1.8.0

Posted by Nowell, Cameron on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Stability-issues-with-LAS-AF-1-8-0-tp590963.html

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Howdy List,
                 We have a leica SP5 system with the leica super Z peizo stage that we recently had upgraded to version 1.8.0 of LAS AF. After the upgrade we ran a test expeirment over night (multiple stage positions and different z stacks) and it ran perfectly. Since then it has crashed about 15-20 times in the sapce of 3 days. We had a new super z stage installed at the same time as the upgrade (to correct some drift issues we were having). But i do not think the crashing is due to the super z stage because the software will crash when you use the zoom functions as well or use wide focus instead of galvo.

Is anyone out there running version 1.8.0 of LAS AF and if so have you had similar issues. We have now downgraded the software back to 1.7.0 and it seems to be running smoothly again. I just need to know if this is a global problem with ver 1.8.0 or it is something specific to our instrument. The leica technitian took 5 goes to get version 1.8 installed as it kept failing at different parts of the firm ware upgrade process. Similarly when taking it back to ver 1.7 i ahd the same problems.

On another note does anyone out there with a super z stage have it vibrate on them? Both stages we have had vibrate and emit a high pitched wine. If you poke it it will stop and everthing is fine. If it is vibrating when you are trying to capture an image then you don't get a good picture.

Thanks

Cam

Cameron Nowell B. Sc (Hons)
Microscopy Imaging and Research Core Facility
Peter MacCallum Cancer Centre
7 St Andrews Place
East Melbourne, Victoria 3002
AUSTRALIA
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