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Mercury lamp alignment

Posted by Dmitry Sokolov-2 on Nov 14, 2007; 1:04am
URL: http://confocal-microscopy-list.275.s1.nabble.com/Stability-issues-with-LAS-AF-1-8-0-tp590963p590966.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear All,

May I ask you for your suggestion on proper procedure of mercury arc bulb alignment of fiber-coupled 106 z lamp housing. I was recommended to align it by maximum of intensity of light passing through the objective of (Leica DM6000B) microscope . The intensity and uniformity is controlled looking on a sheet of paper placed under turret with objective removed.

The corresponding "Leica DM6000B DM6000M Operating manual" has a warning of lamp explosion by overheating if "The bright tips of the arcs, the focal sports..." projected onto each other (p.55) or contact elements of lamp. The alignment procedure is based on viewing the arcs through a special window of the microscope. In my case I see no images of arcs but a number of rings originated probably from the fiber ends.

Should I better remove the fiber coupler and align the lamp looking on it's image on the wall?

I would highly appreciate it if you could share your experience.

Cheers,
Dmitry

___________________________________________
Dr. Dmitry Sokolov,
Manager of Confocal Microscopy Unit
Institute of Molecular Biosciences
Riddet Road, Massey University
Private Bag 11-222, Palmerston North
Tel: +64(6)356-9099 ext. 5549
Email: [hidden email]
Booking: http://confocal-manawatu.pbwiki.com/Booking