Re: Mercury lamp alignment
Posted by
Andrew Resnick on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Stability-issues-with-LAS-AF-1-8-0-tp590963p590967.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Dmitry,
Here's what I do, not sure if it's "factory approved":
First, align the bulb and reflected image of the bulb. Do this by
removing the fiber coupler and projecting the light on a far wall.
The two images of the bulb (direct and reflected) should both be in focus
and side-by-side. Do not overlap the bulb images!
Next, add the fiber coupler, and adjust it (the fiber mount) until the
maximum amount of light exits the fiber.
Finally, do a final adjustment by attaching the fiber coupler to the
microscope and one of the following:
1) focus onto a fluorescent slide (Chroma makes a nice set) and adjust
the fiber/microscope coupling to get a uniform field of illumination
2) Use the special window you mention below. A smoked glass
side-looking mirror is in place in the fluorescent turret to reflect the
illumination to the side port.
3) Project the illumination normally, but without an objective.
Adjust to get a uniform illumination pattern.
I prefer to use #1, as it's more sensible.
Hope this helps...
Andy
At 08:04 PM 11/13/2007, you wrote:
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear
All,
May I ask you for your suggestion on proper procedure of mercury arc bulb
alignment of fiber-coupled 106 z lamp housing. I was recommended to align
it by maximum of intensity of light passing through the objective of
(Leica DM6000B) microscope . The intensity and uniformity is controlled
looking on a sheet of paper placed under turret with objective
removed.
The corresponding "Leica DM6000B DM6000M Operating manual" has
a warning of lamp explosion by overheating if "The bright tips of
the arcs, the focal sports..." projected onto each other (p.55) or
contact elements of lamp. The alignment procedure is based on viewing the
arcs through a special window of the microscope. In my case I see no
images of arcs but a number of rings originated probably from the fiber
ends.
Should I better remove the fiber coupler and align the lamp looking on
it's image on the wall?
I would highly appreciate it if you could share your experience.
Cheers,
Dmitry
___________________________________________
Dr. Dmitry Sokolov,
Manager of Confocal Microscopy Unit
Institute of Molecular Biosciences
Riddet Road, Massey University
Private Bag 11-222, Palmerston North
Tel: +64(6)356-9099 ext. 5549
Email:
[hidden email]
Booking:
http://confocal-manawatu.pbwiki.com/Booking
Andrew Resnick, Ph. D.
Instructor
Department of Physiology and Biophysics
Case Western Reserve University
216-368-6899 (V)
216-368-4223 (F)