Re: Stability issues with LAS AF 1.8.0

Posted by Michael Weber-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Stability-issues-with-LAS-AF-1-8-0-tp590963p590970.html

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Dear Cam,

we are running v1.8 since a couple of weeks and I have to say that this
is the first one which hasn't crashed so far. We had more problems with
v1.6 and v1.7. So it might be an electronics problem on your side. I
also know people still having the same crashing issues with every version.

We had the issue of vibrating stage once during the one year we have the
system, similar to your description. The maximum allowed load for this
stage is quite low (100g?), so I have the suspicion that this vibration
issue could be due to overloading. We also have an older SP2 with Galvo
Stage and this is out of calibration from time to time, probably also
because of overload - this one is an upright stand, so as soon as you
hit the sample with the objective it's for sure more than 100g...

cheers,
Michael


Nowell, Cameron wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Howdy List,
>                  We have a leica SP5 system with the leica super Z peizo
> stage that we recently had upgraded to version 1.8.0 of LAS AF. After
> the upgrade we ran a test expeirment over night (multiple stage
> positions and different z stacks) and it ran perfectly. Since then it
> has crashed about 15-20 times in the sapce of 3 days. We had a new super
> z stage installed at the same time as the upgrade (to correct some drift
> issues we were having). But i do not think the crashing is due to the
> super z stage because the software will crash when you use the zoom
> functions as well or use wide focus instead of galvo.
>
> Is anyone out there running version 1.8.0 of LAS AF and if so have you
> had similar issues. We have now downgraded the software back to 1.7.0
> and it seems to be running smoothly again. I just need to know if this
> is a global problem with ver 1.8.0 or it is something specific to our
> instrument. The leica technitian took 5 goes to get version 1.8
> installed as it kept failing at different parts of the firm ware upgrade
> process. Similarly when taking it back to ver 1.7 i ahd the same problems.
>
> On another note does anyone out there with a super z stage have it
> vibrate on them? Both stages we have had vibrate and emit a high pitched
> wine. If you poke it it will stop and everthing is fine. If it is
> vibrating when you are trying to capture an image then you don't get a
> good picture.
>
>
> Thanks
>
>
> Cam
>
>
> *Cameron Nowell* B. Sc (Hons)
> Microscopy Imaging and Research Core Facility
> Peter MacCallum Cancer Centre
> 7 St Andrews Place
> East Melbourne, Victoria 3002
> AUSTRALIA
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