Posted by
George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Bandpass-filter-kit-and-10-position-filter-wheel-tp590971p590972.html
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalHi Shalin,
Sutter's Lambda-10 series fits your needs, though not your price
(price list is at www.sutter.com). They have controllers for 1, 2 or
3 wheels, and 1, or more, shutters. The Sutter products can be
controlled by most software packages.
Prior Instruments and Applied Scientific Instruments sell wheels, but
probably not at the price point you want. You already mentioned Ludl.
With respect to ThorLabs, two 6-position wheels, in series, each with
one empty position = 10 filter positions, but at the risk of vignetting.
Chroma, Omega and Semrock have plenty of filters.
You can look at SBIG (www.sbig.com) to see if they have any 10
position wheels, may hit your price point but you may have a lot of
work to get their macro language to work with your system.
If this is on a motorized Zeiss stand, ask your local rep about
Zeiss' "Pinkel" wheel. Price point may be close to what you want.
Yuval Garini and Ken Castleman had articles in Cytometry discussing
(ok, arguing) the merits of narrow vs wide filter based approaches.
Having worked with Yuval, I lean toward his suggestion of 'wider is
better'. This would also cover a wider spectral range, i.e. from the
new blue to far-red fluorescent proteins. You may also want to take a
look at Michael Speicher's 8-color M-FISH paper(s), since that is
probably the record for the number of fluorophores in a light
microscope experiment. Both SKY (Yuval) and M-FISH (Castleman and
Speicher) have been unmixing 24 or more combinatorial targets for a decade.
At 02:41 AM 11/14/2007, you wrote:
>Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear all,
>
> A labmate of mine is contemplating bandpass filter-set (25nm FWHM
> with center wavelengths of 450 to 675nm at intervals of 25 nm) for
> doing spectral unmixing.
>
>To go with the filters he is looking for 10 position motorized
>wheel. Preferably the filters (and wheel adapters) should have 1"
>diameter, but smaller diameter is also usable. CVI does provide
>bandpass filters with 25nm FWHM but only at 50nm intervals (whereas
>we need 25nm interval). We are yet to hear about custom filters for
>in-between center wavelengths.
>
>Are there any other off-the-shelf high transmission filter sets
>available from other vendors with said bandwidths?
>
>For filter wheel, we got a quote from Ludl for 10 positions wheel of
>around 5.5K USD. Thorlabs is selling 6 position filter wheel at
>approx. 1K USD. But unfortunately they do not have 10 position
>wheels. Ludl filter wheels are switchable in 50ns whereas thorlabs'
>in 300ms. But he doesn't need real high speeds. Any other vendors of
>10 position wheels that cost say, 1.5K?
>
>Thanks for your suggestions.
>Commercial responses are welcome off the list.
>
>Regards,
>Shalin
>
>
>
>--
>My co-ordinates:
>Shalin Mehta, Graduate student
>Graduate Programme in Bioengineering, NUS, Singapore
>Mobile: +65 90694182
George McNamara, Ph.D.
University of Miami, Miller School of Medicine
Image Core
Miami, FL 33010
[hidden email]
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305-243-8436 office
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